PH9386 | 甘露醇氯化钠琼脂培养基 Mannitol sodium chloride AGAR medium

用途：用于金黄色葡萄球菌的选择性分离培养（中国药典）。

成分（g/L）

胰酪胨 5.0

动物组织胃蛋白酶水解物 5.0

牛肉浸出粉 1.0

D-甘露醇 10.0

氯化钠 75.0

酚红 0.025

琼脂 15.0

pH7.4±0.2  25℃

检验原理：

胰酪胨和牛肉浸出粉提供碳源、氮源、维生素和矿物质；D—甘露醇为可发酵的糖类；较高含量的氯化钠提供较高的渗透压，抑制大多数非葡萄球菌的微生物；酚红为pH 指示剂；琼脂是培养基的凝固剂。典型致病性葡萄球菌（凝固酶阳性）发酵D—甘露醇产酸而产生黄色带有黄晕的菌落，典型非致病性葡萄球菌不发酵D—甘露醇而形成红色菌落。

用法：

称取本品111g，加入1000ml纯化水中，加热溶解并不停搅拌煮沸1分钟，121℃高压灭菌15分钟，备用。

常温运输保存，有效期3年

Usage: For selective isolation and culture of Staphylococcus aureus (Chinese Pharmacopoeia).

Component (g/L)

Peptone from pancreas 5.0

Pepsin hydrolysate from animal tissues 5.0

Beef extract 1.0

D-mannitol 10.0

Sodium chloride 75.0

Phenol red 0.025

Agar 15.0

pH 7.4 ± 0.2 at 25℃

Principle of the test:

Peptone from pancreatic enzymes and beef extract provide carbon sources, nitrogen sources, vitamins and minerals; D-mannitol is a fermentable sugar; a higher content of sodium chloride provides a higher osmotic pressure, which inhibits most non-staphylococcal microorganisms; phenol red is a pH indicator; agar is the solidifying agent of the medium. Typical pathogenic staphylococci (coagulase positive) ferment D-mannitol to produce acid and form yellow colonies with a yellow halo, while typical non-pathogenic staphylococci do not ferment D-mannitol and form red colonies.

Usage:

Weigh 111g of this product, add 1000ml of purified water, heat and dissolve while stirring constantly for 1 minute, boil for 1 minute, sterilize at 121℃ under high pressure for 15 minutes, and set aside for later use.