PH9388 | 乳糖蛋白胨培养基 Lactose Peptone Broth

用途：用于饮用水，水源水中总大肠菌群的测定。

成分（g/L）

蛋白胨 10.0

牛肉浸粉 3.0

乳糖 5.0

氯化钠 5.0

溴甲酚紫 0.016

PH值7.3±0.1 25℃

检验原理：蛋白胨、牛肉浸粉提供碳氮源及矿物质；乳糖是可发酵的糖类；氯化钠维持均衡的渗透压；溴甲酚紫为pH指示剂，酸性呈黄色，碱性呈紫色。

用法：称取本品23.0g，加热溶解于1000ml蒸馏水中，分装到有倒立发酵管的试管中，115℃高压灭菌20分钟，备用。

Application: It is used for the determination of total coliform bacteria in drinking water and source water.

Component (g/L)

Peptone 10.0

Beef extract 3.0

Lactose 5.0

Sodium chloride 5.0

Bromocresol purple 0.016

pH 7.3 ± 0.1 at 25℃

Principle of test: Peptone and beef extract powder provide carbon, nitrogen sources and minerals; lactose is a fermentable sugar; sodium chloride maintains an equilibrium osmotic pressure; bromocresol purple is a pH indicator, showing yellow in acidic conditions and purple in alkaline conditions.

Usage: Weigh out 23.0g of this product, heat and dissolve it in 1000ml of distilled water, then distribute it into test tubes with inverted fermentation tubes, and sterilize at 115℃ under high pressure for 20 minutes. Set aside for later use.